Biodegradability in Seawater (OECD 306)
Marine Biodegradability in Seawater – Closed Bottle Method (OECD 306)
This method is appropriate for volatile materials and although good water solubility is desired, poorly soluble materials can be tested. The test vessels are closed filled bottles containing the test substance dissolved in the natural seawater. The bottles are incubated in the dark at 20°C and the degradation is followed by analysis of the dissolved oxygen over a 28 day period.