Daphnia magna Reproduction (OECD 211)
Daphnia magna Reproduction Test - 21 Day - Semi-static
The primary objective of the test is to assess the effect of chemicals on the rate of reproduction of Daphnia magna. The concentrations used in the study are based on the results of an acute immobilisation test. The duration of the study is 21 days and the number of offspring produced, together with adult and juvenile survival, is reported. A semi-static system is recommended and the frequency of test media renewal depends on the stability of the substance. The study is started with juveniles which are female and start to produce live young by parthenogenesis after about 7 days.
Juvenile production is compared to that of the controls to determine the lowest observed effect concentration (LOEC) and the no effect concentration (NOEC) in addition of the EC50. Other adverse effects are recorded such as abnormal development of juveniles in the brood pouch (white eggs). Shedding of un-hatched eggs, presence of male Daphnia, ephippial eggs and differences in the size of adults at the end of the test are also recorded.