Freshwater Fish Bioconcentration (OECD 305)

Freshwater Bioconcentration: Flow-through Fish Test

The preferred freshwater species are rainbow trout (Oncorhynchus mykiss) and carp (Cyprinus carpio).

The objective of this study is to determine the steady-state bioconcentration factor (BCFss). Fish are exposed under flow-through conditions to water of known quality to which the test substance has been added at known concentrations for a period normally not exceeding 28 days (uptake phase). After the uptake phase the remaining fish are transferred to clean tanks under flow-through conditions for a further period of time normally not exceeding 14 days (depuaration phase). Samples of fish and water are analysed for the test material at predetermined time points in both test phases.


Use this test to determine the steady-state bioconcentration factor (BCFss) of your product in the fish in the freshwater environment.

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