Lemna Growth Inhibition (OECD 221)
Freshwater - Lemna minor Growth Inhibition Test
Cultures of the floating aquatic plant Lemna minor are exposed to a range of concentrations of test substance or effluent under defined conditions light and temperature. The inhibition of growth, relative to unexposed controls, is determined in a static, or semi-static system over 7 days. The data are used to determine the 7 day LC50 (the concentration causing a 50% inhibition of growth rate as measured by the rate of increase in frond numbers) and the NOEC for growth rate. Effects such as chlorosis, abnormal colony formation and root formation are also recorded.