In this test, adult Corophium are exposed to spiked natural sediments for 10 days. During this period, burrowing behaviour may be assessed by counting the number of amphipods on the sediment surface or actively swimming. At the end of the experiment, the amphipods are sieved from the sediment and the number of surviving animals recorded.

Survival is analysed statistically, comparing the controls with the treatments. The objective is to determine the initial concentration, which, in 10 days, kills 50% of the exposed animals (10d LC50).