OECD Biodegradation Screening (OECD 301E)
Freshwater Modified OECD Screening test (OECD 301E / EC C.4.2)
The loss of dissolved organic carbon (DOC) is followed by analysis every few days during the 28 day study period and is compared with the total organic carbon for the known weight of test substance. A sodium acetate reference is used and greater than 60% loss is expected.
This method is similar to the DOC Die-Away Test (OECD 301A) but employs a relatively low concentration of micro-organisms.