Alga Growth Inhibition (ISO 10253)
Marine 72hr EC50 Algal Growth Inhibition Study
Marine Algae (Phaeodactylum tricornutum)
The Alga growth inhibition test ISO 10253 in the marine environment employs the species Phaeodactylum tricornutum (Chemex preferred species) and Skeletonema costatum.
The growth inhibition test is performed semi-axenically (i.e. minimising contamination by other organisms) in glass flasks under controlled conditions. Growth of the alga is determined by cell counting or spectrophotometry at 24-hour intervals for periods of exposure up to 96 hours. The data are used to determine the LC50 value for growth-rate. The highest no effect concentration (NOEC) is determined using appropriate statistical procedures.