Biodegradation - Closed Bottle Test (OECD 301D)

Freshwater Closed Bottle Test (OECD 301D, EC C.4.4)

This Freshwater method is appropriate for volatile materials and although good water solubility is desired, poorly soluble materials can be tested. As implied by the title, this is conducted in a closed system and oxygen is consumption measured by electrochemical probes or through chemical analysis. The principle is similar to the Modified MITI test and suitable controls are used.


This test can be used to look at the biodegradability of both soluble and poorly soluble volatile materials in the freshwater environment.

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