DOC Die-Away Test (OECD 301A / EC C.4.3)
Freshwater Biodegradation - DOC Die-Away Test
The loss of dissolved organic carbon (DOC) is followed by analysis every few days during the 28 day study period and is compared with the total organic carbon for the known weight of test substance. A sodium acetate reference is used and greater than 60% loss is expected.
This method is similar to the Modified OECD Screening Test (OECD 301E) but uses a higher concentration of micro-organisms.